Centre for Stem Cell Research

Paper Publication

• Elizabeth Vinod, Kawin Padmaja , Boopalan Ramasamy, Solomon Sathishkumar. Systematic review of articular cartilage derived chondroprogenitors for cartilage repair in animal models. Journal of Orthopaedics. https://www.sciencedirect.com/science/article/abs/pii/S0972978X22002501 (Accepted: 24th October; Online: November 3^rd)

Invited Talk:

• Saravanabhavan Thangavel. Title of the talk: Genome engineering for therapeutic applications at C VIT, Vellore on 16th Nov. 2022

Paper Publication

• Bagchi, A.; Niveditha, D.; Karthik, C.; Rajediran, V; Venkatesan, V; Sayed, N; Pai, A,A; Nath, A.; David, E; Nakaura, Y; Srivastava, A; Thangavel, S; Murugesan, K,M; Velayudhan, S.R. Erythroid-lineage-specific lentiviral RNAi vectors suitable for molecular functional studies and therapeutic applications. Manuscript accepted in Scientific Reports, Springer Nature. https://www.nature.com/articles/s41598-022-13783-0

Patent Filed

• MohankumarK. Murugesan, Alok Srivastava, Nivedhitha D, Vignesh R. A METHOD FOR ELEVATION OF GAMMA GLOBIN, Patent Application No.: 202241055876, Filed on 29 September 2022 
• COMPOSITIONS AND METHODS FOR TREATING A ß-THALASSEMIA DISEASE. U.S. Pat. App. No. 63/251,229:  David I.K. Martin, Mark DeWitt,Mark C. Walters,Wendy J. Magis, Saravanabhavan Thangavel and Dario Boffelli, filed on October 1, 2022.

Event

• Annual cell and gene therapy symposium, 1-3 September, 2022
• CSCR- Scientific Advisory Committee meeting (SAC), 10th Sep, 2022

Invited talk

• Sonam Pandey. Title of the talk: Research and grant management in Indian Institutions at Charotar University of Science & Technology (CHARUSAT) CHARUSAT Campus-Changa, Anand, Gujarat (India) on 16th Sep. 2022

Paper Publication

• Kawin Padmaja, Soosai Manickam Amirtham, Grace Rebekah, Solomon Sathishkumar, Elizabeth Vinod. Supplementation of articular cartilage-derived chondroprogenitors with bone morphogenic protein-9 enhances chondrogenesis without affecting hypertrophy. Biotechnology Letters. 3rd  August 2022 (https://link.springer.com/article/10.1007/s10529-022-03280-9).
• Venkatesan V, Christopher A, Karuppusamy K, Babu P, Azhagiri M and Thangavel S. CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications. JoVE. August 9, 2022 doi: 10.3791/64064.

Paper Accepted

• Bagchi, A.; Niveditha, D.; Karthik, C.; Rajediran, V; Venkatesan, V; Sayed, N; Pai, A,A; Nath, A.; David, E; Nakaura, Y; Srivastava, A; Thangavel, S; Murugesan, K,M; Velayudhan, S.R. Erythroid-lineage-specific lentiviral RNAi vectors suitable for molecular functional studies and therapeutic applications. Manuscript accepted in Scientific Reports, Springer Nature.

Patent Filed

• Srujan Marepally, Alok Srivastava. FORMULATIONS, LIPID COMPOUND, METHODS AND THEREOF, PCT Application No.: PCT/IN2022/050660, Filed on 22-July-2022.
• Shaji R Velayudhan, Alok Srivastava, Mohankumar K. Murugesan. A METHOD FOR DOWNREGULATION OF A TARGET GENE AND A COMPOSITION THEREOF. Indian Patent Application No. 202241045242 filed on 8 Aug 2022.

Invited Talk

• Thangavel S. Genome Engineering of human hematopoietic stem and progenitor cells for gene therapy applications. August 6, 2022. Bharath institute of higher education and research, Chennai.

Paper accepted:  

1. Kawin Padmaja, Soosai Manickam Amirtham, Grace Rebekah., Solomon Sathishkumar, Elizabeth Vinod. Supplementation of articular cartilage-derived chondroprogenitors with Bone Morphogenic Protein-9 enhances chondrogenesis without affecting hypertrophy. Biotechnology Letters (06.07.2022)
2. Vigneshwaran Venkatesan, Abisha Crystal Christopher, Karthik V. Karuppusamy, Prathibha Babu1,2, Manoj Kumar K. Alagiri1,2, Saravanabhavan Thangavel. CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications. JOVE

Paper publication: 

1. Vigneshwaran Venkatesan, Abisha Crystal Christopher, Prathibha Babu, Manoj Kumar K Azhagiri, Kaivalya Walavalkar, Bharath Saravanan, Saranya Srinivasan, Karthik V Karuppusamy, Annlin Jacob, Sumathi Rangaraj, Abhirup Bagchi, Aswin Anand Pai, Yukio Nakamura, Poonkuzhali Balasubramanian, Rekha Pai, Srujan Kumar Marepally, Kumarasamypet Murugesan Mohankumar, Shaji R Velayudhan,  View ORCID ProfileDimple Notani, Alok Srivastava,  View ORCID ProfileSaravanabhavan Thangavel Editing the core region in HPFH deletions alters fetal and adult globin expression for treatment of β-hemoglobinopathies.  doi: https://doi.org/10.1101/2022.07.11.499646

Events held: 

1. Workshop conducted jointly by CSCR, Evident (Olympus), and DSS on “Confocal & Multiphoton Microscopy” on 14th and 15th July 2022.This workshop was organized by Dr Sandya Rani, Scientist-D (CSCR Core Facility).

Paper publication: 

1. Hubert D-J Daniel, Sanjay Kumar, Rajesh Kannangai, Farzana J, Joseph N Joel, Aby Abraham, Kavitha M Lakshmi, Mavis Agbandje-McKenna, Kirsten E Coleman, Arun Srivastava, Alok Srivastava, Asha M Abraham. Age-stratified adeno-associated virus serotype 3 neutralizing and total antibody prevalence in hemophilia A patient from India. J Med Virol. 2022 May 16. Online ahead of print.  PMID: 35577570 DOI: 10.1002/jmv.27859

Preprint Publication: 

1. Prasad K, Devaraju N, George A, Ravi NS, Mahalingam G, … Mohankumar KM* et al. Precise modelling and correction of a spectrum of β-thalassemic mutations in human erythroid cells by base editors [Internet]. bioRxiv; 2022 [cited 2022 Jun 13]. p. 2022.06.01.494256. Available from: https://www.biorxiv.org/content/10.1101/2022.06.01.494256v1

Provisional Patent Application filed:

1. Mohankumar K. Murugesan, Alok Srivastava, Kirti Prasad. A METHOD FOR MODIFICATION OF ß-GLOBIN GENE. Indian Patent Application No. 202241030885 filed on 30-05-2022
2. Saravanabhavan Thangavel, Alok Srivastava. A METHOD FOR REACTIVATION OF FETAL HEMOGLOBIN AND A COMPOSITION THEREOF. Indian Patent Application No. 202241030465 filed on dated 27-05-2022.

Events: 

Below mentioned events was coordinated by Mr. Rajesh A., Technical Officer- Core Facilities:

1. Training on Biorad Chemidoc Data analysis software and a Road Show conducted by Biorad India team; Date: 14/06/2022.
2. Presentation on usage of Beckman Ultracentrifuge and high speed centrifuges by Biorad India team; Date: 15/06/2022.
3. Real time Demo on the operation and maintenance of Beckman Ultracentrifuge and High speed centrifuges and rotors by Biorad India team; Date:16-06-2022.

Paper Publication

• Devaraju N, Rajendiran V, Ravi NS, Mohankumar KM. Genome Engineering of Hematopoietic Stem Cells Using CRISPR/Cas9 System. Methods Mol Biol. 2022;2429:307-331. doi: 10.1007/978-1-0716-1979-7_20. PMID: 35507170
• Precilla S , Kuduvalli S ,  Praveena E , Thangavel S and T S Anitha. Integration of synthetic and natural derivatives revives the therapeutic potential of temozolomide against glioma- an in vitro and in vivo perspective.  Life Sci. 2022 May 6; 301:120609. doi: 10.1016/j.lfs.2022.120609.

Patent

• COMPOSITIONS AND METHODS FOR TREATING A ß-THALASSEMIA DISEASE. U.S. Pat. App. No. 63/251,229: David I.K. Martin, Mark DeWitt, Mark C. Walters,Wendy J. Magis, Saravanabhavan Thangavel and Dario Boffelli.

Paper Publication

• Noel Naveen Johnson, Soosai Manickam Amirtham, B. Sandya Rani, Solomon Sathishkumar, Grace Rebekah, and Elizabeth Vinod. Assessment of the inherent chondrogenic potential of human articular cartilage-derived chondroprogenitors in pellet culture using a novel whole pellet processing approach. Journal of Orthopaedics, March 2022. https://doi.org/10.1016/j.jor.2022.03.007.

Invited Talk

• Event: International bone marrow failure syndrome.
  Title of the talk: Preferential expansion of primitive hematopoietic stem cells for gene therapy applications organized by CMC Vellore on 8th April 2022 (Saravanabhavan Thangavel).
• Event: Special Seminar Series
  Title of the talk: Genome engineering for therapeutic applications organized by School of life sciences, VIT Vellore on 11th April 2022 (Saravanabhavan Thangavel).
• Event: RUSA 2.0, Genome Editing Seminar Series
  Title of the talk: Genome engineering human hematopoietic stem and progenitor cells for gene therapy applications organized by Univ. of Madras on 12th April, 2022 (Saravanabhavan Thangavel).

Paper Accepted

• Hithavan R, Shireesha M, Porkizhi A, Vigneshwaran V, Saravanabhavan T, Srujan M, Srilakshmi P, Influence of Hydrophobicity in Hydrophilic Region of Cationic Lipids on Enhancing Nucleic Acid Delivery and Gene Editing, ACS Applied Bio Materials (Co-corresponding author)
• Hari Krishnareddy Rachamalla, Chandrashekhar Voshavar, Porkizhi arjunan, Gokulnath Mahalingam, Rashmi Praksash Chowath, Rajkumar Banerjee*, Praveen Kumar Vemula, Srujan Marepally Skin permeable nano-lithocholic-lipidoid efficiently alleviates psoriasis-like chronic skin inflammations, ACS Applied Materials and Interfaces.

Paper Published

• Karuppusamy K, Demosthenes J, Venkatesan V, Christopher A, Babu P, Azhagiri M, Jacob A, Ramalingam V, Rangaraj S, Mohankumar K.M, Marepally S, Varghese G, Srivastava A, Kannangai R and Thangavel S. The CCR5 gene-edited CD34+CD90+ Hematopoietic stem cell population serves as an optimal graft source for HIV gene therapy. Front. Immunol., 14 March 2022
• Ravi NS, Wienert B, Wyman SK, Bell HW, George A, Mahalingam G, Vu JT, Prasad K, Bandlamudi BP, Devaraju N, Rajendiran V, Syedbasha N, Pai AA, Nakamura Y, Kurita R, Narayanasamy M, Balasubramanian P, Thangavel S, Marepally S, Velayudhan SR, Srivastava A, DeWitt MA, Crossley M, Corn JE, Mohankumar KM.. Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin. Elife. 2022 Feb 11;11:e65421. doi: 10.7554/eLife.65421. PMID: 35147495; PMCID: PMC8865852.

Invited Talk

• Title of the talk: Recent trends in stem cell application, organized by Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai on 2nd March, 2022 (Saravanabhavan Thangavel)