Centre for Stem Cell Research

Paper Accepted

• Elizabeth Vinod, Noel Naveen Johnson, Sanjay Kumar, Soosai Manickam Amirtham, Jithu Varghese James, Abel Livingston, Grace Rebekah, Alfred Job Daniel, Boopalan Ramasamy and Solomon Sathishkumar. Migratory chondroprogenitors retain superior intrinsic chondrogenic potential for regenerative cartilage repair as compared to human fibronectin derived chondroprogenitors”. Scientific reports, Nature. (Date: 16.11.2021)

Events Held

• Centre for Stem Cell Research (a Unit of inStem), CMC campus, Bagayam, Vellore and Becton Dickinson (BD) Biosciences, have jointly conducted two days Hands on and virtual module workshop on “Advanced Flowcytometry and High Dimension Data analysis” from 1st to 2nd November, 2021. This workshop was coordinated by Dr Sandya Rani, Scientist-D (CSCR Core Facility).

Papers Published

• Elizabeth Vinod, Kawin Padmaja, Abel Livingston, Jithu Varghese James, Soosai Manickam Amirtham, Solomon Sathishkumar, Boopalan Ramasamy, Grace Rebekah, Alfred Job Daniel, UpasanaKachroo.Prospective isolation and characterization of chondroprogenitors from human chondrocytes based on CD166/CD34/CD146 surface markers.CARTILAGE. September 2021. doi:10.1177/19476035211042412.

Papers Published

• Abisha Crystal Christopher, Vigneshwaran Venkatesan, Karthik V Karuppusamy, Saranya Srinivasan, Prathibha Babu, Manoj Kumar K Azhagiri, Karthik C, Abhirup Bagchi, Vignesh Rajendiran, Nithin Sam Ravi, Sanjay Kumar, Srujan Kumar Marepally, Kumarasamypet Murugesan Mohankumar, Alok Srivastava, Shaji R Velayudhan, Saravanabhavan Thangavel. Preferential expansion of human CD34+CD133+CD90+ hematopoietic stem cells enhances gene-modified cell frequency for gene therapy. Hum Gene Ther. 2021 Sep 5. doi: 10.1089/hum.2021.089. Online ahead of print.
• Manoj Kumar K. Azhagiri, Prathibha Babu, Vigneshwaran Venkatesan & Saravanabhavan Thangavel. Homology-directed gene-editing approaches for hematopoietic stem and progenitor cell gene therapy. Stem Cell Research & Therapy. 12; 500 (2021). https://doi.org/10.1186/s13287-021-02565-6.

Scientific talk

• Dr. Amit Kumar Bhattacharya, Technical Promotion Consultant from TissueGnostics, Gurgaon has delivered a talk on “Tissue FAXS: possibilities with cytometry in the spatial context of the tissue” on 16th Sep. 2021 at CSCR (virtual). This Scientific talk is arranged by Dr Sandya Rani, Scientific Officer (CSCR Core Facility).

Papers Published

• Jannu AK, Puppala ER, Gawali B, Syamprasad NP, Alexander A, Marepally S, Chella N, Gangasani JK, Naidu VGM. Lithocholic acid-tryptophan conjugate (UniPR126) based mixed micelle as a nano carrier for specific delivery of niclosamide to prostate cancer via EphA2 receptor. J Pharm. 2021 Jun 22;605: 120819. doi: 10.1016/j.ijpharm.2021.120819.

Papers Accepted

• Elizabeth Vinod, Kawin Padmaja, Abel Livingston, Jithu Varghese James, Soosai Manickam Amirtham, Solomon Sathishkumar, Boopalan Ramasamy, Grace Rebekah, Alfred Job Daniel, Upasana Kachroo. Prospective isolation and characterization of chondroprogenitors from human chondrocytes based on CD166/CD34/CD146 surface markers. Cartilage. (Date of acceptance: 03.08.2021).

Papers Published

• Roshni Parameswaran, Upasana Kachroo, Soosai Manickam Amirtham, Grace Rebekah, Elizabeth Vinod. An invitro analysis of the effect of hyperosmolarity on the chondrogenic potential of human articular cartilage derived chondroprogenitors. Tissue and Cell. Volume 72, October 2021, 101590

Papers accepted

• Soosai Manickam Amirtham, Upasana Kachroo, Deepak Vinod Francis, Kawin Padmaja and Elizabeth Vinod. (2021) An improved method for processing chondroprogenitor pellets following chondrogenic differentiation for histology and immunohistochemical staining using agarose. Journal of Arthroscopy and Surgery. https://doi.org/10.1016/j.jajs.2021.05.005

Paper Accepted

• Gokulnath Mahalingam, Aruna Mohan, Porkizhi Arjunan, Ajay Kumar Dhyani, Kanimozhi Subramaniyam, Yogapriya Periyasamy, Srujan Marepally, Lipid nanoparticle enabled delivery of chemically modified mRNA into mammalian cells, JOVe (June 2021, accepted)

Papers published

• Madhuri Vrisha, Ramesh Sowmya, Raymond Renita, Selina Agnes, Loganathan, Lakshmi. "Translational Research in Osteogenesis Imperfecta and Cell Therapy" 2021; Proceedings 72,1: 3. (10^th May 2021)  
• Ramesh Sowmya, Dolly Daniel, Cecilia Götherström, and Vrisha Madhuri. "Trophic effects of multiple administration of mesenchymal stem cells in children with osteogenesis imperfecta." Clinical and Translational Medicine 11, no. 4 (2021): e385. (4^th April 2021)

Invited talks:/h5

Dr. Srujan Kumar Marepally

• Delivered a lecture on "Development of lipid based nucleic acid therapeutics: Understanding the structural parameters of the lipids towards improving the transfection efficiencies" in a webinar conducted on Science beyond boundary: Invention, Discovery, Innovation and Society, 30th April 2021.

Award:

• Dr. Marepally has received award Bronze medal from Chirantan Rasayan Sanstha, India, 30^th April,2021

Paper Accepted

• Elizabeth Vinod,Roshni Parameswaran, Soosai Manickam Amirtham, Grace Rebekah, Upasana Kachroo. Comparative analysis of human bone marrow mesenchymal stem cells, articular cartilage derived chondroprogenitors and chondrocytes to determine cell superiority for cartilage regeneration. Acta Histochemica. 6th April 2021.
• Elizabeth Vinod, Soosai Manickam Amirtham, Upasana Kachroo, Anjali Goyal, Ozlem Ozbey, Jithu Varghese James, Solomon Sathishkumar, Boopalan Ramasamy. Articular chondroprogenitors in platelet rich plasma for treatment of osteoarthritis and osteochondral defects in a rabbit knee model. The Knee. 18th March 2021.

Paper published

• Elizabeth Vinod, Soosai Manickam Amirtham, Upasana Kachroo, Anjali Goyal, Ozlem Ozbey, Jithu Varghese James, Solomon Sathishkumar, Boopalan Ramasamy. Articular chondroprogenitors in platelet rich plasma for treatment of osteoarthritis and osteochondral defects in a rabbit knee model. The Knee. 13th April 2021.
• Karthik V Karuppusamy , Prathibha Babu , Saravanabhavan Thangavel. The Strategies and Challenges of CCR5 Gene Editing in Hematopoietic Stem and Progenitor Cells for the Treatment of HIV. Stem Cell Rev Rep. 31st March 2021[PMID: 33788143 DOI: 10.1007/s12015-021-10145-7].

Paper Accepted: 

• Karthik V Karuppasamy, Prathibha Babu and Saravanabhavan Thangavel. The Strategies and Challenges of CCR5 Gene Editing in Hematopoietic Stem and Progenitor Cells for the Treatment of HIV". Stemcell reviews and reports. Accepted on 15 March 2021.
• Elizabeth Vinod, Soosai Manickam Amirtham, Upasana Kachroo. Effect of human articular chondroprogenitor derived conditioned media on chondrogenic potential of bone marrow derived mesenchymal stromal cells. The Knee. Accepted on 16 February 2021.  
• Elizabeth Vinod, Kawin Padmaja, Upasana Kachroo. Journal of Orthopaedics, Trauma and Rehabilitation. Accepted on 8 March 2021.  
• Devaraju N, Rajendran V, Ravi NS , Mohankumar KM. Genome engineering of hematopoietic stem cells using CRISPR-Cas9 system. Methods in Molecular Biology.  Accepted on Feb, 2021.

Published: 

• Elizabeth Vinodab, Soosai Manickam Amirthama, Upasana Kachroo. An assessment of bone marrow mesenchymal stem cell and human articular cartilage derived chondroprogenitor cocultures vs. monocultures. The Knee Volume 29, March 2021, Pages 418-425.
• Abhirup Bagchi, Aneesha Nath,Vasanth Thamodaran, Smitha Ijee, Dhavapriya Palani, Vignesh Rajendiran, Vigneshwaran Venkatesan, Phaneendra Datari , Aswin Anand Pai, Nancy Beryl Janet, Poonkuzhali Balasubramanian, Yukio Nakamura , Alok Srivastava, Kumarasamypet Murugesan Mohankumar, Saravanabhavan Thangavel, Shaji R. Velayudhan. Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells. Cells 2021, 10, 523. https://doi.org/10.3390/cells1003052 .

Paper Accepted:

•   Gurbind Singh, Kannan V Manian, Chitra Premkumar, Alok Srivastava, Dolly Daniel and Shaji R Velayudhan. Derivation of clinical-grade induced pluripotent stem cell lines from erythroid progenitor cells in xenofree conditions.”[Accepted: Methods in molecular biology].
• Vasanth Thamotharan, Sonam Rani and Shaji R Velayudhan.“Gene editing in human induced pluripotent stem cells using doxycycline-inducible CRISPR-Cas9 system” [Accepted: Methods in molecular biology]
• Venkanna Muripitia, Brijesh Lohchania, Venkatesh Ravulaa, Shireesha, Manturthi, Srujan Marepally, Amarnath Velidandi, Srilakshmi V. Patri, Dramatic influence of the hydroxy functionality of azasugar moiety in the head group region of tocopherol-based cationic lipids on in vitro gene transfection efficacies​ (Accepted in New Journal of Chemistry)

Published:

• Manipulation of Developmental Gamma-Globin Gene Expression: an Approach for Healing Hemoglobinopathies. Vigneshwaran Venkatesan, Saranya Srinivasan, Prathibha Babu and Saravanabhavan Thangavel. Molecular and cellular biology, Jan., 2021.

Invited Talks:

• Title of the talk: "Stem cell-based approaches for studying red cell diseases"
  Name of the symposium/conference: Virtual International Conference "Update on Stem Cells –Academic and Industrial Perspectives – Future Challenges" conducted on 24th December 2020 at VIT.