Centre for Stem Cell Research

Papers Accepted:

• Jesi Charles, Latha Nedumaran, Swetha Raman, Elizabeth Vinod, Rajalakshmi Rajasegaran, Kamalakannan Vadivel, Anand Bhaskar, Sathya Subramani. Rhythm generating mechanisms in rat sinoatrial node. Current Medical Issues.10th November 2023.

Papers Published:

• Deepak Vinod Francis, Aishwarya Jayakumaran Rajeswari, John Bino Stephen, Ganesh Parasuraman, Jeya Lisha J Abel Livingston, Sandya Rani, Alfred Job Daniel, Solomon Sathishkumar, Elizabeth Vinod. An ultrastructural report of human articular cartilage resident cells in correlation with their phenotypic characteristics. Journal of Histotechnology. 26th October 2023. https://www.tandfonline.com/doi/full/10.1080/01478885.2023.2278118.
• Ganesh Parasuraman, Elizabeth Vinod, Abel Livingston, Solomon Sathishkumar and Deepak Vinod Francis. Comparative assessment of the morphology and antigenicity of human osteochondral units using formalin and coagulant fixative. European Journal of Anatomy. 6th November 2023. https://doi.org/10.52083/CVZF3774.

Invited Talks:

• 9th Congress of Indian Cartilage Society, Chennai on “Understanding cell- based Cartilage reconstruction”. Session: Orthobiologics in chondral pathologies: Enhancing Healing Potential. (Dr Elizabeth Vinod)

Awards:

• Dr Elizabeth Vinod received these Two awards
  1.  Rev. Fr. Lourdu Yeddanapalli Award, 2023, Christian Medical College, Vellore 13.11.20232. ICS Felicitation award at the 9th Congress of Indian Cartilage Society, Chennai for poster presentation at the International Cartilage Regeneration & Joint Preservation Society, 17th World Congress at Sitges/Barcelona, Spain on 20.10.2023

Papers Accepted:

• Balaji Balakrishnan, Raveen Stephen Stallon Illangeswaran, Bharathi Murugan Rajamani, Arun Kumar Arunachalam, Aswin Anand Pai, Ezhilpavai Mohanan, Alok Srivastava, Vikram Mathews, Poonkuzhali Balasubramanian. Metformin pretreatment ameliorates busulfan induced liver endothelial toxicity during haematopoietic stem cell transplantation
  (Accepted for publication in PlosOne, October 2023)
• Aswin Anand Pai, Ezhilpavai Mohanan, John C Panetta , Uday P. Kulkarni, Stallon Illangeswaran RS, Balaji Balakrishnan, Agila Jayaraman, Eunice S Edison, Kavitha ML, Anup J. Devasia, Fouzia NA, Anu Korula, Aby Abraham, Biju George, Alok Srivastava, Vikram Mathews, Joseph F Standing, Poonkuzhali Balasubramanian. Treosulfan Exposure Predicts Thalassemia-free Survival In Patients With Beta Thalassemia Major (TM) Undergoing Allogeneic Hematopoietic Cell Transplantation (Accepted for publication in Clinical Pharmacology and Therapeutics October 2023)

Paper Published

• Ramya Murugesan, Karthik V. Karuppusamy, Srujan Marepally, Saravanabhavan Thangavel1*. Current approaches and potential challenges in the delivery of gene editing cargos into hematopoietic stem and progenitor cells. Front. Genome Ed., 15 September 2023. Sec. Genome Editing in Blood Disorders Volume 5 - 2023 | https://doi.org/10.3389/fgeed.2023.1148693

Event:

• Stem Cell awareness program organized on 13th October, 2023. Total participants: 95 (5 colleges)

Patent:

• A complete patent application titled “A METHOD FOR ELEVATION OF GAMMA GLOBIN” has been filed at the Indian Patent Office (IPO). Application No: 202241055876; Dated: 29-Sep-23. inventor(s): Mohankumar K. Murugesan, Alok Srivastava, Nivedhitha D.

Invited Talk:

• Dr Srujan Marepally delivered a lecture on" Delivery of mRNA with Lipid nanoparticles, in vitro and in vivo" in mRNA Vaccine and Therapeutics Discussion Meeting, held on 21 sept 2023, at Indian Institute of Science (IISc) Bengaluru.

Papers Accepted:

• Nithin Sam, Anila George, Mohankumar K M*. Arrayed gRNA screening by base editors in mammalian cell lines using lentiviral system. STAR-PROTOCOLS-D-23-00427R1.

Poster Presentation:

Poster presentation on International Cartilage Regeneration & Joint Preservation Society, 17th World Congress at Sitges/Barcelona, Spain from September 9th to September 12th, 2023

• Elizabeth Vinod, Ganesh Parasuraman, Abel Livingston, Soosai Manickam Amirtham, Grace Rebekah, Jeya Lisha J, Alfred Job Daniel, Solomon Sathishkumar. Poster presentation titled:- “A Comparative Study of Techniques for Isolation and Culture of Human Migratory Chondroprogenitors from Articular Cartilage
• Elizabeth Vinod, Ganesh Parasuraman, Jeya Lisha J, Soosai Manickam Amirtham, Abel Livingston, Jithu James Varghese, Sandya Rani, Deepak Vinod Francis, Grace Rebekah, Alfred Job Daniel, Boopalan Ramasamy, Solomon Sathishkumar. Poster presentation titled:- “Fetal cartilage-derived chondrocytes and chondroprogenitors display superior chondrogenic potential than adult resident cartilage cells”.

Event:

• 8th annual cell and cell therapy symposium held from 31st Aug to 2nd Sep, 2023.

Papers Accepted:

• Sipra Rout , Soosai Manickam Amirtham , Mythraeyee Prasad , Anne George Cherian , Sandya Rani B ,Yesudas Sudhakar , Neetu Prince,In Vitro Human Fetal Pancreatic Islets to Redefine Pancreatic Research Journal: Cureus. 2023 Rout et al. Cureus 15(8): e43244. DOI 10.7759/cureus.4324.

Event:

• Hands on Workshop on Multicolor Advanced Flow Cytometry was conducted on 1st and 2nd Aug 2023 at CSCR, supported by Beckman Coulter Life Sciences. Dr. H. Krishnamurthy from NCBS has given a talk on the Basics of Flow cytometry as a part of the workshop. 

Number of participants: 22

Coordinated by Dr. Sandya Rani.B 

• Biosafety workshop was conducted on 14th Aug, 2023 at CSCR.

Name of participants- 48

Coordinated by Dr. Gurbind Singh and Dr Sonam Pandey

Papers Accepted:

• Soosai Manickam Amirtham, Ganesh Parasuraman, Jeya Lisha J, Deepak Vinod Francis, Abel Livingston, Grace Rebekha, Solomon Sathishkumar and Elizabeth Vinod. Decellularized Fetal Collagen Exhibits Chondroinductive Potential for Bone Marrow-Derived Mesenchymal Stem Cells by Enhancing Glycosaminoglycan Production. European Journal of Anatomy. 8th July 2023.
• Sonam Rani, Vasanth Thamodaran, Krittika Nandy, Fouzia Nambiathayil Aboobacker, Madhavi Maddali, Praveena Rajesh, S Vijayanand, Ernest David, Shaji Ramachandran Velayudhan. Establishment and characterization of CSCRi006-A: An induced pluripotent stem cell line generated from a patient with Diamond-Blackfan Anemia (DBA) carrying ribosomal protein S19 (RPS19) mutation. (https://doi.org/10.21203/rs.3.rs-2876104/v1); Ms. No. HUCE-D-23-00336R2

Event:

• Technical presentation conducted online at CSCR on "Muvicyte (live-cell imaging system)" from Revvity (Formerly known as PerkinElmer) on 14th July 2023, Coordinated by Sandya Rani. B, CSCR. Speaker: Mr.Eby John, Application Manager, PerkinElmer India Pvt Ltd

Number of participants: 21

Papers Published

• Esther Sathya Bama Benjamin, Dinesh Babu, Gaurav Joshi, Bharathi, M Rajamani, Krittika Nandy, Sonam Rani, Senthamizhselvi Anandan, Chitra Premkumar, MadhaviMaddali, Aby Abraham,  Shaji R Velayudhan, Poonkuzhali Balasubramanian.Inhibition of BCR::ABL1 tyrosine kinase activity Aids in the Generation of Stable Chronic Myeloid Leukemia Induced Pluripotent Stem Cells(https://doi.org/10.1101/2023.06.01.543015). preprint

Papers Accepted:

• Ganesh Parasuraman, Elizabeth Vinod, Abel Livingston, Solomon Sathishkumar and Deepak Vinod Francis. Comparative assessment of the morphology and antigenicity of human osteochondral units using formalin and coagulant fixative European Journal of Anatomy Accepted on: 21.05.2023

Papers Published

• Vigneshwaran V., Abisha C.C., Manuel R., Manoj KA., Prathibha B, Kaivalya W., Bharath S., Geoffroy A, Sumathi R, Saranya S, Karthik V. K.,Annlin J, Abhirup B, Aswin A.Pai, Yukio N. , Ryo K, Poonkuzhali B, Rekha P., Srujan M., Mohankumar KM., Shaji R. V., Melanie B., Dimple N, Toni C., Alok S. Saravanabhavan T., Editing the core region in HPFH deletions alters fetal and adult globin expression for treatment of b-hemoglobinopathies. Molecular Therapy Nucleic Acids. Published: April 26, 2023 DOI:https://doi.org/10.1016/j.omtn.2023.04.024.
• Elizabeth Vinod, Ganesh Parasuraman, Abel Livingston, Soosai Manickam Amirtham, Grace Rebekah, Jeya Lisha J, Alfred Job Daniel, Solomon Sathishkumar. Comparison of methods for the isolation and culture of Migratory Chondroprogenitors from Human articular cartilage. Connective Tissue Research. Date: April 24, 2023.
• Elizabeth Vinod, Ganesh Parasuraman, Jeya Lisha J, Soosai Manickam Amirtham, Abel Livingston, Jithu James Varghese, Sandya Rani, Deepak Vinod Francis, Grace Rebekah, Alfred Job Daniel, Boopalan Ramasamy, Solomon Sathishkumar. Human fetal cartilage-derived chondrocytes and chondroprogenitors display a greater commitment to chondrogenesis than adult cartilage resident cells. Plos One. Published Date: April 27, 2023.

Papers Accepted:

• Gokulnath M, Porkizhi A, Yogapriya P, Ajay D, Nivedita D, Vignesh R, Abhisha C, Ramya Devi KT2, Immanuel D, Saravanabhavan T, Mohankumar M, Mahesh M, Alok S, Srujan M*., Correlating the differences in the receptor binding domain of SARS-CoV-2 spike variants on their interactions with human ACE2 receptor. Scientific Reports (Accepted)

Event:

• An outreach Program was conducted for the Post Graduate students from the Biotechnology department, Thiruvalluvar University, Serkadu, Tamil Nadu on 21st April 2023 at CSCR, Vellore. Basic Scientific talks and practical demonstrations were conducted by Dr. Saravanabhavan Thangavel, Dr. M. Mohankumar, and Dr. Sandya Rani. B from CSCR, Vellore. Number of Participants – 15. (Program coordinated by Dr. Saravanabhavan Thangavel)

Papers Published

• Alok Srivastava, Savita Rangarajan, Kaan Kavakli, Robert Klamrot, Gili Kenet, Liane Khoo, Chur-Woo You, Weiqun Xu , Niel Malan, Laurent Frenzel, Catherine N Bagot, Oleksandra Stasyshyn, Chia-Yau Chang, Stacey Poloskey, Zhiying Qiu, Shauna Andersson, Baisong Mei and Steven W Pipe . Fitusiran prophylaxis in people with severe haemophilia A or haemophilia B without inhibitors (ATLAS-A/B): a multicentre, open-label, randomised, phase 3 trial.Lancet Haematol . 2023 Mar 29;S2352-3026(23)00037-6.  DOI:https://doi.org/10.1016/S2352-3026(23)00037-6.
• Guy Young, Alok Srivastava, Kaan Kavakli, Cecil Ross, Jameela Sathar, Chur-Woo You, Huyen Tran, Jing Sun, Runhui Wu, Stacey Poloskey, Zhiying Qiu, Salim Kichou, Shauna Andersson, Baisong Mei, Savita Rangarajan. Efficacy and safety of fitusiran prophylaxis in people with haemophilia A or haemophilia B with inhibitors (ATLAS-INH): a multicentre, open-label, randomised phase 3 trial. The Lancet. Published online: March 29, 2023. DOI:https://doi.org/10.1016/S0140-6736(23)00284-2.

Papers Accepted:

• Elizabeth Vinod, Ganesh Parasuraman, Abel Livingston, Soosai Manickam Amirtham, Grace Rebekah, Jeya Lisha J, Alfred Job Daniel, Solomon Sathishkumar. “Comparison of methods for the isolation and culture of Migratory Chondroprogenitors from Human articular cartilage”Connective Tissue Research . Accepted date: April 6, 2023
• Elizabeth Vinod, Ganesh Parasuraman, Jeya Lisha J, Soosai Manickam Amirtham, Abel Livingston, Jithu James Varghese, Sandya Rani, Deepak Vinod Francis, Grace Rebekah, Alfred Job Daniel, Boopalan Ramasamy, Solomon Sathishkumar. Human fetal cartilage-derived chondrocytes and chondroprogenitors display a greater commitment to chondrogenesis than adult cartilage resident cells. Plos One. Accepted date: April 17, 2023
• Vigneshwaran Venkatesan, Abisha Crystal Christopher, Manuel Rhiel, Manoj Kumar K Azhagiri, Prathibha Babu, Kaivalya Walavalkar, Bharath Saravanan, Geoffroy Andrieux, Sumathi Rangaraj, Saranya Srinivasan, Karthik V Karuppusamy, Annlin Jacob, Abhirup Bagchi, Aswin Anand Pai, Yukio Nakamura, Ryo Kurita, Poonkuzhali Balasubramanian, Rekha Pai, Srujan Kumar Marepally, Kumarasamypet Murugesan Mohankumar, Shaji R Velayudhan, Melanie Boerries, Dimple Notani, Toni Cathomen, Alok Srivastava, and Saravanabhavan Thangavel*. Editing the core region in HPFH deletions alters fetal and adult globin expression for treatment of β-hemoglobinopathies. Molecular therapy nucleic acids.

Invited Talk:

• Genome engineering for hemoglobinopathies" Indira Gandhi Medical College and Research Institute, Puducherry on 8th April 2023. (Dr Mohan KM).

Event:

• IVIS® Spectrum CT and Living Imaging system hands-on training conducted at CSCR by Dr. Shahzada Asad | Senior Product Specialist, Small Animal in vivo Imaging, Perkin Elmer on 6th April 2023. Coordinated by Dr. Vigneshwar and Dr. Sandya Rani. B.
• Technical presentation at CSCR on 14th April 2023 - Presented by Dr. Manimaran Paramasivam, Field Application Specialist- BR, Spinco Biotech Pvt Ltd, Hyderabad.Title: "ImageXpress Confocal HT.ai: A Complete Solution for Automated Imaging and High Content Screening". Coordinated by Dr. Sandya Rani. B.

Papers Published

• Raveen Stephen, Stallon Illangeswaran, Daniel Zechariah Paul Jebanesan , Kezia Kanimozhi Sivakumar , Rakhi Thalayattu Vidhyadharan , Bharathi M. Rajamani , Nancy Beryl Janet , Ernest David , Shaji Ramachandran Velayudhan, Vikram Mathews , Poonkuzhali Balasubramanian. Chemotherapeutic drugs elicit stemness and metabolic alteration to mediate acquired drug-resistant phenotype in acute myeloid leukemia cell lines. Leukemia Research, Volume 128, May 2023. (https://www.sciencedirect.com/science/article/abs/pii/S0145212623000395?via%3Dihub)
• Gaurav Joshi, Nancy Bery, Janet Arthur , Thenral S Geetha , Phaneendra Venkateswara Rao Datari , Kirti Modak , Debanjan Roy , Anurag Dutta Chaudhury , Prasanth Sundaraganesan , Sweety Priyanka , Fouzia Na , Vedam Ramprasad , Aby Abraham , Vivi M Srivastava , Alok Srivastava , Uday Prakash Kulkarni , Biju George , Shaji R Velayudhan. Comprehensive laboratory diagnosis of Fanconi anaemia: comparison of cellular and molecular analysis. J Med Genet. 2023 Mar 9; jmedgenet-2022-108714. doi: 10.1136/jmg-2022-108714. Online ahead of print.

National Science Day

• On February 28, 2023, CSCR had hosted a science awareness program to recognize National Science Day for college students to be exposed to the scope of stem cell and gene therapy research in India.

Papers Published

• Lohchania B, Christopher A, Arjunan P, Mahalingam G, Kathirvelu D, Prasannan A, Venkatesan V, Taneja P, Mohan Kumar Km , Thangavel S , Marepally S ."Diosgenin enhances liposome-enabled nucleic acid delivery and CRISPR/Cas9-mediated gene editing by modulating endocytic pathwaysFront Bioeng Biotechnol. 2023 Jan 9;10:1031049. doi: 10.3389/fbioe.2022.1031049.
• Hemalatha R, Muthuraman N, Sandya Rani, Premila Abraham. A study on the alteration of endoplasmic reticulum stress related proteins in cyclophosphamide induced damage to urothelium. Pharmaceutical and Biomedical research on 31.01.2023.

Invited Talks

• Srujan Kumar Marepally delivered lecture on "Webinar Oligo Virtual Event - Nucleic acids therapeutics delivery by LNPs: Fundamentals and Characterization" conducted by Agilent Technologies on 19th January 2023.
• Saravanabhavan Thangavel delivered lecture on " Genome edited stem cells for therapeutic applications" conducted by Sri Balaji Vidyapeeth, Puducherry (ADHR sponsored training programme) on 10th Feb 2023.

Papers Published

• Brijesh Lohchania, Abisha Crystal Christopher, Porkizhi Arjunan, Gokulnath Mahalingam, Durga Kathirvelu, Aishwarya Prasannan, Vigneshwaran Venkatesan, Pankaj Taneja, Mohan Kumar KM, Saravanabhavan Thangavel* and Marepally S.* Diosgenin enhances liposome-enabled nucleic acid delivery and CRISPR/Cas9-mediated gene editing by modulating endocytic pathways, Front. Bioeng. Biotechnol., Sec. Preclinical Cell and Gene Therapy 2023, doi.org/10.3389/fbioe.2022.1031049
• George A, Ravi NS, Prasad K, Panigrahi L, Koikkara S, Rajendiran V, Devaraju N, Paul J, Pai AA, Nakamura Y, Kurita R, Balasubramanian P, Thangavel S, Marepally S, Velayudhan SR, Srivastava A, Mohankumar KM. Efficient and error-free correction of sickle mutation in human erythroid cells using prime editor-2, Front Genome Ed. 2022 Dec 20;4:1085111. doi: 10.3389/fgeed.2022.1085111
• Mahalingam G, Periyasami Y, Arjunan P, Subaschandrabose RK, Mathivanan TV, Mathew RS, Ramya Devi K, Premkumar PS, Muliyil J, Srivastava A, Moorthy M*, Marepally S*. Omicron infection increases IgG binding to spike protein of predecessor variants. J Med Virol. 2022 Dec 22. doi: 10.1002/jmv.28419.

Paper Presentation

• Dr. Elizabeth Vinod presented an oral paper titled “Comparison of methods for the isolation and culture of Migratory Chondroprogenitors from Human articular cartilage” at the 8th Congress of the Indian Cartilage Society and the 7th Congress of the Asian Cartilage Repair Society held from December 16th to 17th at Jaipur, Rajasthan.