Centre for Stem Cell Research
A unit of inStem, Bengaluru, Christian Medical College Capmus, Bagayam Vellore
Strains
The CSCR-LAF maintains eleven different strains of mice - including knockout and SCID strains and a single strain of rat. The majority of rodent strains are bred under strictly inbred conditions. For the very first time in India, we procured NSG mice, the most immunodeficient strain for our institution from Jackson Laboratory, United States. Animals were quarantined in a prescribed manner; on an observation all the animals were found to be healthy enough. Animals are put under breeding now.
|
S.No |
Strain |
Description |
Disease Model |
Source |
|
1 |
C57BL/6J |
Inbred strain |
Multi- Purpose model |
Jax Lab, US |
|
2 |
BALB c/J mice |
Inbred strain |
Inbred Strain |
Jax Lab, US |
|
3 |
FVB/NCrl mice |
Inbred strain |
Mouse leukemia model |
Charles River, UK |
|
4 |
CD-1 |
Outbred strain |
Sentinel animals, Pseudopregnancy |
Charles River, UK |
|
5 |
B6.129S4-F8tm1Kaz/J |
Mutant Stock; Targeted Mutation |
Hemophilia A |
Jax Lab, US |
|
6 |
B6.129P2-F9tm1Dws/J |
Congenic; Mutant Strain |
Hemophilia B |
Jax Lab, US |
|
7 |
B6.129-Adamts13tm1Dgi/J |
Mutant Stock; Targeted Mutation |
OCT-GFP model |
Jax Lab, US |
|
8 |
B6.129-Adamts13tm1Dgi/J |
Congenic; Mutant Strain |
Thrombotic Thrombo¬cytic Purpura |
Jax Lab, US |
|
9 |
B6.CB17-Prkdcscid/SzJ |
SCID |
Transplantation studies |
Jax Lab, US |
|
10 |
C.B-17/Icr-PrkdcIc-rIcoCrl |
SCID |
Xeno Graft Research |
Charles River, UK |
|
11 |
Sprague-Dawley |
Rat- Outbred strain |
Orthopedic surgery |
Jax Lab, US |
|
12 |
NSG |
SCID |
Immunodeficient |
Jax Lab, US |
Routine/ Conventional Microbiology
Routine sentinel animal sampling is being done in every three months to ensure the health status of breeding and experimental animals stock. Animal skin and hair samples are checked for ectoparasites. Fecal samples are checked for the endoparasites by a sedimentation method. Microbiological examination of animal room air, animal feed, water, bedding material, fecal samples and throat swabs are also being carried out in every month.
ELISA based Microbiology
Furthermore, randomly selected serum samples are screened microbiologically by ELISA-based kits for selected rodent pathogens such as Mouse Pneumonia Virus (MPV), Mouse Lymphocytic Choriomeningitis Virus (MLCV), Mouse Noro Virus (MNV) and Mouse Hepatitis Virus (MHV).
PCR-based Monitoring
Blood samples of sentinel animals are checked for Mycoplasma pulmonis by PCR method.
Genetic Monitoringy
Genetic monitoring of mutant and SCID strains are often conducted by PCR. All report of QC is maintained in CSCR Laboratory Animal Facility.
Protocols established
SOP’s for Subcapsular renal cell transplantation and Retro-orbital injection and timed pregnancy were created.