Centre for Stem Cell Research

Description

The CSCR Flow Cytometry and Cell Sorting Facility provide a broad array of instrumentation, support, education, and consultation to the research community. A wide variety of cell sorting modes are supported, from one-way to four-way tube cell sorting, Plate sorting, Slide sorting using high speed to low speed, different sizes of nozzles with 11 colors, and 13 parameters. Additionally, a wide variety of cell analysis services (up to 19 colors, 21 parameters) are offered. Currently, the facility offers one cell sorter (BD FACS Aria III) and two analyzers (BD FACS Celesta and BC Cytoflex LX) and two computers dedicated to the offline analysis of the flow cytometry data using FlowJo and Kaluza software.

The BD FACSAria III sorter is a superior multicolor performance, and legendary ease-of-use that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve unrivaled sensitivity and resolution. BD FACSAria III cell sorter with a 5 laser 11 colour setup. The BD FACSAria III system has a throughput of 70,000 events per second and can do 4-way sorting and single cell sorting. Wavelength choices include 561-nm (Y/G), as well as the 488-nm (Blue), 633-nm (Red), 405-nm (Violet), and 375-nm (UV) lasers.