iPSC Workshop 2019

Centre for Stem Cell Research (CSCR) conducted its second Human induced Pluripontent Stem Cells (iPSC) workshop Brochure in February 2019 for a period of 6 days from 18th to 23rd.

The workshop involved hand-on-training and lectures by

Odity Mukherjee, Institute for Stem Cell Biology and Regenerative Medicine (inStem), Bengaluru,
Maneesha Inamdar, Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Bengaluru
Sivaprakash Ramalingam, Genomics and Integrative Biology (IGIB), New Delhi
Gurbind Singh, Centre for Stem Cell Research, Tamil Nadu
R.V. Shaji, Centre for Stem Cell Research, Tamil Nadu

Eleven research scholars from different research institutes all over India were selected to participate in the program.

S.No	Participants	Institution
1	Poulomi Adhikari	Indian Institute of Technology Guwahati
2	Soham Jagtap	National Institute of Mental Health and Neurosciences
3	Asha Shaji Antony	Central University of Kerala
4	Syed Shahid Musvi	University of Hyderabad
5	Athira Sajan	Central University of Kerala
6	Shivani Sharma	National Institute of Mental Health and Neurosciences
7	Porkizhi	Centre for Stem Cell Research
8	Harsimar Bhatt	National Institute of Mental Health and Neurosciences
9	Kamini Gautam	Institute for Stem Cell Biology and Regenerative Medicine
10	Vignesh R	Centre for Stem Cell Research
11	Gloria Narayan	Indian Institute of Technology Guwahati

The workshop objectives were to provide knowledge and training in:

Various steps involved in the derivation of iPSCs
Cryopreservation
Thawing of iPSC cell line
Expansion and passaging in feeder-free conditions
Characterization of iPSC by using various molecular biology techniques such as RNA isolation, cDNA synthesis, RT-PCR, immunofluorescence, Flow cytometry.
Embryoid body formation
Differentiation of iPSC into endoderm mesoderm ectoderm lineages.

Photos from the 2nd iPSC workshop

Cell Culture Laboratory

Centre for Stem Cell Research

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